Cytotoxicity test of Betel extract using Brine Shrimp Lethality Assay

Abstract

Piper betle, recognized for its potential antimicrobial and antifungal properties, presents an intriguing avenue for investigating its efficacy in cancer treatment. This study aims to determine the cytotoxic effects of Piper betle extract utilizing the Brine Shrimp Lethality Assay (BSLA). The results showed that when the concentration of Piper betle extract increased, the survival time of nauplii decreased in a concentration-dependent manner. Additionally, a dose-response connection was seen in the mortality rate, with greater concentrations translating into higher mortality. Significant variations in cytotoxicity between the Piper betle extract and the negative control were validated by statistical analysis. The finding is evident because it indicates that Piper betle extract has cytotoxic properties, which may have consequences for cancer research. Although more research is necessary to clarify the underlying mechanisms and pinpoint particular bioactive chemicals, the findings emphasize how critical it is to investigate Piper betle's potential for oncology therapy.